Small Wonder-Lyzer


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Home arrow Applications arrow Enzyme Immobilization Studies

Enzyme Immobilization Studies

Even though Enzyme Immobilization has been studied since the second half of the 20th century it is still cynosure of todays research. Small Wonder-Lyzer™ owing to its novel design can easily be used for Enzyme Immobilization Studies. Practically every enzyme can be successfully immobilized using SWL™.

Theory

When enzyme solution is poured into the membrane sac, the enzyme gets entrapped owing to small pore size of the membrane (10-12 kD). Though enzyme cannot come out, substrate freely diffuses into the sac, interacts with the enzyme, gets converted into product and diffuses out into the surrounding solution. This process continues as long as a concentration gradient exists between substrate and enzyme. Once all substrate is converted to product, equilibrium will be reached and process will stop. At this stage, the membrane unit containing the entrapped enzyme can simply be lifted and placed into contact with new substrate! Thus, enzyme does not get lost and can be used again and again. Also, there is no contamination of the final product with the enzyme.

Advantages of using SWL™

Membrane has good mechanical properties and chemical stability. Enzyme by this process remains very comfortably protected and is not subjected to any mechanical shear or stress.  Enzyme does not contaminate the final product and can easily be reused again and again.

Comparison of membrane aided immobilization with SWL™ immobilization

Features
General
SWL™
Preparation
Simple
Simple
Binding force
Strong
None
Enzyme leakage
No
No
Applicability
Very wide
Very wide
Matrix effects
No
No
Large diffusional barriers
Yes
No
Microbial protection
Yes
Yes


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